 | Actonians Association | 1977- | Joined from the Nemean Amateur League Old Actonians Association until 2015 |
 | AFC Oldsmiths | 2014- | Joined from South London Alliance |
 | Alexandra Park | 1925-1938 and 1946- | Joined from the Nemean Amateur League |
 | Alleyn Old Boys | 1907- | Founder members |
 | Aquarius | 1911-1959 | Moved to the Nemean Amateur League |
| Ashford | 1921-1924 | ||
 | Bank of England | 1920- | |
 | Barclays Bank | 1920-2015 | became BB Eagles in 2002 |
 | BB Eagles | 1920-2015 | Barclays Bank until 2002 Merged with Ibis in 2015 to form Ibis Eagles |
 | Borough Polytechnic | 1946-2001 | Became Polytechnic of the South Bank in 1973 |
| Bowes Park | 1907-1913 | Founder members | |
 | Brentham | 1946-2003 | Moved to the Middlesex County League |
 | Britannic House | 1928-1933 and 1962-1982 | Merged with Lensbury as Lensbury & Britannic House from 1933-1962 Renamed British Petroleum in 1982 |
| British Petroleum | 1982-1991 | Britannic House until 1982 |
 | Broomfield | 1958- | Joined from the Nemean Amateur League |
 | Cambridge Heath | 2017- | Joined from the West End London AFA |
 | Cambridge Town | 1913-1935 | Moved to the Spartan League |
 | Carshalton | 1912- | |
 | Casuals | 1907-1914 | Founder members Moved to the Isthmian League |
 | Catford Wanderers | 1929-1982 | Moved to the Southern Olympian League |
 | CGU Cuaco | 1999-2001 | Cuaco until 1999 Merged with South Bank AFC in 2002 to form South Bank Cuaco |
| Cheshunt | 1907-1921 | Founder members | |
 | City of London | 2017- | Joined from the Amateur Football Combination |
 | Civil Service | 1907-1914 and 1928- | Founder members |
 | Corinthian-Casuals | 2017- | |
 | Crouch End Vampires | 1907-1914 and 1928- | Founder members |
| Croydon | 1907-1908 | Founder members | |
| Cuaco | 1919-1999 | renamed CGU Cuaco in 1999 |
 | Ealing Association | 1907-1970 | Founder members Moved to the Nemean Amateur League |
 | East Barnet Old Grammarians | 1977- | Joined from the Old Boys League |
 | East Grinstead | 1928-1935 | Moved to the Mid-Sussex League |
 | Eastbourne | 1907-1946 | Moved to the Corinthian League |
 | Guys Hospital | 1948-1953 | |
| Hampstead | 1907-1914 | Founder members Merged with Richmond in 1921 to form Hampstead & Richmond | |
| Hampstead & Richmond | 1921-1922 | ||
 | Hampstead Heathens | 2017- | Joined from the Amateur Football Combination |
 | Hastings & St. Leonards | 1927-1946 | Moved to the Corinthian League |
| Highgate | 1919-1931 and 1936-1939 | Moved to the Southern Olympian League | |
 | HMS Excellent | 1935-1936 | |
 | Honourable Artillery Company | 1919-1920 | |
| HSBC | 1921- | London Joint City & Midland Bank 1921-1923 until Midland Bank 1923-1998 | |
| Ibis | 1921-2015 | Merged with BB Eagles in 2015 to form Ibis Eagles |
 | Ibis Eagles | 2015- | |
 | Ipswich Town | 1907-1935 | Joined the Eastern Counties League |
 | Kew Association | 1925- | Joined from the Southern Olympian League |
 | London County Westminster & Parr's Bank | 1911-1970 | Merged with National Provincial Bank in 1970 to form National Westminster Bank |
 | London Joint City & Midland Bank | 1921- | Renamed Midland Bank in 1923 |
| Lee | 1907-1910 | ||
 | Lensbury | 1927-1933 and 1962- | Joined from the Southern Olympian League Merged with Britannic House as Lensbury & Britannic House from 1933-1962 Renamed Weirside Rangers in 2002 |
| Lensbury & Britannic House | 1933-1962 | De-merged to form Britannic House A.F.C. and Lensbury A.F.C in 1962 | |
 | Lloyds AFC | 1923-2013 | Merged with The Warren JFC in 2014 to form Lloyds Warren |
 | Lloyds Bank | 1923-2013 | Renamed Lloyds TSB Bank in 1999 |
| Lloyds TSB Bank | 1923-2013 | Lloyds Bank until 1999 Renamed Lloyds AFC in 2013 |
 | Lloyds Warren | 2013- | |
 | London Lions | 2017- | |
 | Merton | 1919- | |
| Midland Bank | 1921- | Renamed HSBC in 1998 |
 | Motor Union | 1924-1930 | |
 | National Provincial Bank | 1924-1970 | Merged with Westminster Bank in 1970 to form National Westminster Bank |
 | National Westminster Bank | 1970-2002 | Moved to the London Financial F.A. |
| New Crusaders | 1907-1913 | Founder members Moved to the Isthmian League | |
 | Norsemen | 1908- | |
 | Nottsborough | 2002- | Joined from the Southern Olympian League |
 | NUFC Oilers | 2013- | Joined from the West End London AFA |
| Old Actonians Association | 1977- | Joined from the Nemean Amateur League Renamed Actonians Association in 2015 |
 | Old Blues | 2011- | Played one season as Associate Members |
 | Old Bromleians | 1967-2002 | Joined from the Southern Olympian League Moved to the Kent County League |
 | Old Esthameians | 1971-2016 | Joined from the Southern Olympian League Moved to the Essex Alliance League |
 | Old Finchleians | 1998- | Joined from the Old Finchleians |
 | Old Garchonians | 2011- | Played one season as Associate Members |
 | Old Grocers | 1931-1938 | Moved to the Old Boys League |
 | Old Haberdashers | 1919-1924 | |
 | Old Latymerians | 1945-2013 | Joined from the Spartan League |
 | Old Lyonians | 1924- | |
 | Old Owens | 1996- | Joined from the Southern Olympian League |
 | Old Parkonians | 1919- | Joined from the Old Boys League |
 | Old Parmiterians | 1992-2004 | Joined from the Southern Olympian League Moved to the Amateur Football Combination |
 | Old Salesians | 1983- | Joined from the Old Boys League |
 | Old Stationers | 1932- | Joined from the Old Boys League |
 | Old Westminster Citizens | 1935- | Joined from the Old Boys League |
 | Old Wilsonians | 2004- | Joined from the Amateur Football Combination |
| Oxford | 1910-1913 | ||
 | Pearl Assurance | 1936-1989 | Joined from the Southern Olympian League Relocated to Peterborough |
 | Pinner | 1951-1961 | Moved to the Southern Olympian League |
 | Polytechnic | 1954- | Joined from the Spartan League |
 | Polytechnic of the South Bank | 1946-2001 | Borough Polytechnic until 1973 Renamed South Bank Polytechnic in 1975 |
 | Reigate Priory | 1907-1997 | Founder members |
| Richmond Association | 1907-1914 | Merged with Hampstead in 1921 to form Hampstead & Richmond | |
 | Royal Bank Of Scotland | 1990-1994 | Joined from the London Banks F.A. Moved to the London Financial F.A. |
| Snaresbrook | 1920-1921 | ||
 | Somers Town Future | 2017- | |
| South Bank AFC | 1946-2001 | South Bank Polytechnic until 1995 Merged with CGU Cuaco in 2002 to form South Bank Cuaco |
 | South Bank Cuaco | 2001- | |
| South Bank Polytechnic | 1946-2001 | Polytechnic of the South Bank until 1975 Renamed South Bank AFC in 1995 |
 | Southgate Olympic | 1958-2015 | Joined from the Southern Olympian League Moved to the Amateur Football Combination |
 | St. James' Old Boys | 2015- | |
 | Tansley | 2017- | |
| The Warren | 2013- | Lloyds Warren until 2015 |
 | Thornycrofts Athletic | 1933-1939 | Moved to the Hampshirea League |
 | Toc H. | 1925-1932 | Joined from the Southern Olympian League |
| Townley Park | 1907-1914 | ||
 | Tunbridge Wells | 1908-1911 | |
| Weirside Rangers | 1927-1933 and 1962- | Lensbury until 2002 | |
 | West Wickham | 1938- | |
| Westminster Bank | 1911-1970 | London County Westminster & Parr's Bank until 1922 Merged with National Provincial Bank in 1970 to form National Westminster Bank |
| Weybridge | 1907-1908 and 1913-1914 | ||
 | Winchmore Hill | 1928- | Joined from the Southern Olympian League |
